Coding

Part:BBa_K4119056:Design

Designed by: Chenchen Xu   Group: iGEM22_NJTech_China   (2022-10-11)


pRADK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1227
    Illegal XbaI site found at 1231
    Illegal PstI site found at 1242
    Illegal PstI site found at 1279
    Illegal PstI site found at 3139
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1227
    Illegal NheI site found at 2294
    Illegal PstI site found at 1242
    Illegal PstI site found at 1279
    Illegal PstI site found at 3139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1227
    Illegal BglII site found at 1191
    Illegal BglII site found at 4406
    Illegal BamHI site found at 1265
    Illegal XhoI site found at 1187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1227
    Illegal XbaI site found at 1231
    Illegal PstI site found at 1242
    Illegal PstI site found at 1279
    Illegal PstI site found at 3139
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1227
    Illegal XbaI site found at 1231
    Illegal PstI site found at 1242
    Illegal PstI site found at 1279
    Illegal PstI site found at 3139
    Illegal NgoMIV site found at 5070
    Illegal AgeI site found at 1658
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 937
    Illegal SapI.rc site found at 2433
    Illegal SapI.rc site found at 4919
    Illegal SapI.rc site found at 5129


Design Notes

none.


Source

pRADK backbone plasmid Deinococus and E.Coli shuttle plasmid

References